Direct SPR-MS Analysis on a Single Biochip2013-05-13
In collaboration with the CLIPP proteomic platform, HORIBA Scientific offer you the opportunity to identify and quantify many spatially resolved samples in a multiplex format.
The CLIPP platform and HORIBA Scientific, strengthened by recent progress in SPRi-MS coupling, are working together for the clinical validation of a SPRi-MS platform. From this strategic partnership a CIFRE research fellow has been contracted, and the following benefits are expected: technological advances in nano/micro-technologies adapted to biochips, automation of on-chip MS analyses and development of bio-statistical tools dedicated to this platform.
- Label-free detection of biomolecular interactions
- Multiplexed binding characterization
- Simple transfer of the SPRi micro-array biochip to MS (no elution step)
- Direct MS and MS/MS analysis on the SPRi micro-array biochip
MS analysis is directly performed on the SPRi biochip thanks to our compatible proprietary surface chemistry (developed with LAMBE, UMR 8587 CNRS). The CS surface chemistry avoids non-specific binding on the surface of the biochip and facilitates subsequent MS analysis.
The SPRi-PlexII™ system provides automated monitoring of up to 400 biomolecular interactions occurring upon the functionalized surface of the SPRi Biochip™. The interaction between the ligands and the analytes can be studied rapidly and reliably with minimal user intervention.
A video clip describing MultiPlex label-Free interaction analysis platform is found on Gammadata’s homepage.
Additional information is found on HORIBA’s dedicated web page for Label-free Biomolecular Interaction Analysis using Surface Plasmon Resonance Imaging